Three Ways Music Can Help You To Lose Weight
Music is the key to the soul. It can lift you up and put a smile on your face or alternatively it can bring you down and reduce you to tears. Music is powerful and fortunately you can use it to help with your weight loss. (more…)

Know Your Numbers
When you are trying to lose weight, focus is usually on the pounds you weigh, the pounds you have already lost, the pounds you have still to lose, your measurements and possibly your body fat percentage. However, your goal should not just be about losing weight and inches, it should be about leading a healthier […] (more…)

Characterization of a monoclonal antibody with specificity for holo-transcobalamin
Background:
Holotranscobalamin, cobalamin-saturated transcobalamin, is the minor fraction of circulating cobalamin (vitamin B12), which is available for cellular uptake and hence is physiologically relevant. Currently, no method allows simple, direct quantification of holotranscobalamin. We now report on the identification and characterization of a monoclonal antibody with a unique specificity for holotranscobalamin.
Methods:
The specificity and affinity of the monoclonal antibodies were determined using surface plasmon resonance and recombinant transcobalamin as well as by immobilizing the antibodies on magnetic microspheres and using native transcobalamin in serum. The epitope of the holotranscobalamin specific antibody was identified using phage display and comparison to a de novo generated three-dimensional model of transcobalamin using the program Rosetta. A direct assay for holotrnscobalamin in the ELISA format was developed using the specific antibody and compared to the commercial assay HoloTC RIA.
Results:
An antibody exhibiting >100-fold specificity for holotranscobalamin over apotranscobalamin was identified. The affinity but not the specificity varied inversely with ionic strength and pH, indicating importance of electrostatic interactions. The epitope was discontinuous and epitope mapping of the antibody by phage display identified two similar motifs with no direct sequence similarity to transcobalamin. A comparison of the motifs with a de novo generated three-dimensional model of transcobalamin identified two structures in the N-terminal part of transcobalamin that resembled the motif. Using this antibody an ELISA based prototype assay was developed and compared to the only available commercial assay for measuring holotranscobalamin, HoloTC RIA.
Conclusion:
The identified antibody possesses a unique specificity for holotranscobalamin and can be used to develop a direct assay for the quantification of holotranscobalamin. (more…)

Teen Diet Online Weight Loss and Parenting NewsCur…
Teen Diet Online Weight Loss and Parenting NewsCurrent Weight Loss News Research shows that the entire diet, not just the vitamin, must be considered This is fascinating research about the importance of looking at the big picture when researchers studying nutritional supplements, vitamins and minerals. In this study researchers were able to demonstrate that the absorbability of vitamin (more…)

Dieting Preparation — The Impact of Sleep & Stress
Dieting PreparationThe Impact of Sleep and StressWhen you have started a diet in the past, how much sincere and attentive focus did you give to preparing for your diet? Isn’t it true that usually you are getting the food together a particular diet requires,… (more…)

How to Lose Weight Healthily and Keep Fats Off Permanently
Why do people lose weight and then to gain the fats back so soon? Do you want to lose fat permanently? Here is how to keep off the fats forever! (more…)

Low-Fat Diet Has Little Effect on Reducing Risk of Breast Cancer
Newswise - A major study that includes nearly 50,000 women followed over 8 years indicates that a diet low in fat, but high in fruit, vegetables and grains, does not significantly reduce the risk of breast … (more…)

CarbWire Feature: The Low-carb Lifestyle: It Works!
Written by Bill Fairbairn. I’ve got one thing to say to those diet pundits who claim the low-carb lifestyle does not work: BS. As in beef steak - a thick, succulent top sirloin grilled to mouth-watering perfection. Yes, those in… (more…)